Respiration and Photosynthesis Lab Report | Photosynthesis

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AP Bio Lab Report for Photosynthesis
  Respiration and Photosynthesis Lab ReportAP BiologyAbstract. By observing how many bubbles arose from the Elodea plant, we kept track of whichsituations it was best suited for and what makes the plant respire(or not respire). Bubbles of oxygen floated to the top as the plants used the light from the lamp and carbon dioxide toperform the necessary process to respire; the process of photosynthesis. Althoughphotosynthesis occurred at all distances from the light source, the amount of bubbles decreasedas we increased the distance from the light source. Intro Cellular respiration and photosynthesis are processes necessary for life organisms, they help tosustain life. Cellular respiration occurs in the mitochondria of the cell, using ATP to releasebroken down byproducts. Photosynthesis goes through a different (and opposite) process tocreate an organic compound like glucose using sunlight converted to chemical energy (ATP).We observed how to calculate the rate of photosynthesis, and observed how light affects thisrate. It also allowed us a better understanding between the differences (and forms) of respiration. Methods. Our lab was conducted in Mrs. Wootton’s biology classroom on November 3, 2011. The main  objective of our lab was photosynthetic rate by observing bubbles from the sea plant, Elodea,while varying distance from light and amount of tap water versus sodium bicarbonate. First, wefilled up four different test tubes with tap water. We then labeled the test tubes numbers 1 to 4.Next, we took a sprig of Elodea and cut it into four separate pieces. We put one piece in each of the test tubes, fully submerging them with their cut end placed upward. We then filled a beakerwith tap water and placed it in front of an ordinary lamp, that we used for our light source. Thebeaker was placed in front to be our insulator from the heat of the lamp. Next, we placed our testtube rack with our test tubes on the other side of the beaker, so that the beaker was in the  middle. We carefully measured out 25 cm from the light for the placement of our test tube rack,for our first run of data collection. Since our Elodea pieces were shocked from the waterplacement and cutting of them we allowed them to sit for five minutes before we started lookingfor oxygen bubbles. After the wait time, bubbles started appearing and we counted the number torecord for our data, for five minutes. Then, we moved the rack to both the 50 cm and 75 cmmarks and repeated the process for each to derive data. For the next section of the lab, we tookall the test tubes and emptied out all the water. We then replaced test tube numbers 1 and 2 withtap water again, while test tubes 3 and 4 received 0.5% sodium bicarbonate. When we replacedeach of our test tubes with fresh water and sodium bicarbonate, we had to keep in mind that wehad to add the water to one rapid and slow bubble producer and the sodium bicarbonate to theother two test tubes. With our new test tubes, we placed them on the rack 25m from the light andwatched for bubbles for five minutes. In the end, we collected our data and analyzed it todiscover the photosynthetic rates of the plant, Elodea, in different substances for a certainamount of time with a light source.
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